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In the development of a portable biosensor-based diagnostic device, one specific material of paramount importance is the microfluidic chip. These chips play a critical role in sample processing and analysis by enabling precise control and manipulation of small volumes of biological samples. Microfluidic chips are essential for enhancing sensitivity and specificity in detecting biomarkers associated with various diseases or pathogens. They facilitate efficient mixing, separation, and detection of target molecules, ultimately improving the device's diagnostic accuracy and reliability. Additionally, microfluidic chips contribute to the device's portability and user-friendliness by minimizing sample volumes and simplifying workflow automation. Thus, the selection and optimization of microfluidic chip materials and designs are crucial for the successful development and performance of a portable biosensor-based diagnostic device. Youtube video : https:yph1YMsz68