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This study developed a hydrogel wound dressing infused with Jatropha podagrica leaf extract to evaluate its antibacterial potential. Saponin-rich extracts were obtained by ethanol extraction and solvent partitioning, then incorporated into gelatin hydrogels at varying concentrations (0–100%v/v). The antibacterial activity was assessed using the disk diffusion method. Results showed that the hydrogel with 80% extract achieved the greatest bacterial inhibition. These findings suggest that Jatropha podagrica extract enhances hydrogel efficacy, highlighting its potential as a natural antibacterial wound dressing.